WP3

Equipment

IIMCB laboratories are equipped with state-of-the-art scientific equipment, including three confocal microscopes (multiphoton Zeiss LSM710 NLO, Zeiss LSM5 Exciter, Leica TCS SP 2 AOBS), Nikon and Olympus fluorescence microscopes, digital imaging equipment, crystallographic equipment (unique in this part of Europe), a mass spectrometry laboratory with MALDI and ESI spectrometers, HPLC, FPLC, surface plasmon resonance Biacore, FACS, real-time PCR, and analytical centrifuge, etc. Consistent with IIMCB’s policy that all major equipment is shared between laboratories, this equipment will be available to the new zebrafish laboratory. However, several large pieces of sophisticated equipment will be needed, such as a state-of-the-art fluorescence microscope system for time-lapse imaging of zebrafish (e.g., single-plane illumination fluorescence microscope), light and fluorescence stereomicroscopes, microinjectors, and a system for the behavioural analysis of larvae and adult zebrafish. Additionally, the Zebrafish Core Facility will be expanded by the purchase of additional pieces of equipment, such as a light stereomicroscope, microbiological incubator, and decontaminator. The acquisition of new equipment and upgrading of other pieces of equipment will allow us to begin working on all of the projects without any limitations.

1. Mikroskop Lightsheet.Z1

IIMCB laboratories are equipped with state-of-the-art scientific equipment. However, several large pieces of equipment have been purchased for zebrafish-related research. The best example is Lightsheet.Z1, state-of-the-art fluorescence microscope manufactured by Zeiss. It works on the principle of single-plane illumination microscopy (SPIM) where the illuminating planar sheet of light is perpendicular to the detection axis. This allows for much more efficient usage of illumination light, thereby reducing the unwanted effects of photobleaching and phototoxicity. The sample, typically embedded in agarose rods, can be moved in all directions as well as rotated 360° degrees during imaging. Lightsheet.Z1 is particularly well suited for long-term live imaging of medium sized objects, such as zebrafish larvae.

List of equipment purchased:
1. Laboratory incubators and PCR: 1 incubator Memmert, 2 incubators, 1 PCR
2. State-of-the-art fluorescence microscope system for time-laps imaging of zebrafish: single-plane illumination microscope
3. Light stereomicroscopes
4. Microinjectors: CellTram Vario, Pipette holder keepers and micromanipulators, Microinjectors, Mounting adaptors
5. Complete system for 96 well adult zebrafish analysis
6. A set-up to electrophoretically analyse protein modifications and stable and transient interactions under controlled temperature and power conditions (DNA pointer)
7. Light stereomicroscope: Light stereomicroscopes, Light stereomicroscope with a camera, System for imaging and data storing
8. Dewar: Cooling cabinet and Freezing cabinet Liebherr, Freezer -80
9. Dishwasher Miele with equipment
10. Laboratory autoclave
11. Bloter
12. Mini trans blot
13. Mini horizontal gel electrophoresis system
14. Laboratory furniture
15. Renovation of a laboratory for the research on zebrafish

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